Human diffuse large B-cell lymphoma|340121 |BNCC

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TMD8

Culture medium	BNCC338360
Description	RPMI-1640 Complete Medium (with 10% FBS)
Composition	90%RPMI-1640+10%FBS
Growth conditions	37 ℃; 5% CO2+95% air;
Subculture procedure	Resuscitation steps: ① Remove the cryovial tube from liquid nitrogen or -80 ℃ freezer and place it in PE gloves, quickly immerse it in a 37 ℃ water bath, shake the cryovial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete culture medium on a super clean bench, centrifuge at 1000-1200rpm for 3-5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete culture medium. ③ Then add the cell suspension to a T25 bottle containing 6-7mL of complete culture medium and culture it in an incubator. It is recommended to cultivate the bottle vertically. Cell passage: ① Centrifuge method: Collect cells, centrifuge at 1000rpm for 5 minutes, discard the supernatant, add 1-2mL of culture medium and blow evenly. Divide the cell suspension into new T25 bottles containing 8mL of culture medium in a ratio of 1:2. ② Half volume liquid change method: half volume liquid change method can be selected; Please gently aspirate half of the supernatant culture medium, resuspend the remaining culture medium with cell sediment, and divide the cell suspension into new T25 bottles containing 8mL of culture medium at a ratio of 1:2. ③ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches greater than 2 × 106 cells/mL.
morphology	Lymphocyte like, round, single cell, mostly clustered
Sharing mode	Public welfare sharing