Rat liver cancer cells|392453 |BNCC

Essential Information Related Products

H4-II-E-C3

Culture medium	BNCC394583
Description
Composition
Growth conditions	37 ℃; 5% CO2+95% air;
Attention	If any abnormality is found on the day of receipt, please contact customer service within 24 hours. Failure to do so will be considered as confirmation of good condition upon receipt.For cryovial format: Upon receipt, immediately store at -80°C. For long-term storage, transfer to liquid nitrogen overnight. During resuscitation, use the entire contents of one vial at a time; do not reserve any portion.For T25 flask format: Upon receipt, let the flask stand in the incubator for 2–3 hours before proceeding with routine cell handling.Please strictly follow the instructions above. Failure to do so may result in cell inactivation, and no replacement will be provided.
Subculture procedure	Resuscitation steps: ① Remove the cryopreservation tube from liquid nitrogen or -80 ℃ refrigerator and place it in PE gloves, quickly immerse it in a 37 ℃ water bath, shake the cryopreservation tube to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete culture medium on a super clean bench, centrifuge at 1000-1200rpm for 5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete culture medium. ③ Add the cell suspension into a T25 bottle containing 5-6mL of complete culture medium and incubate in a culture incubator. Cell passage: ① Remove the old culture medium, wash twice with PBS, and add 1-2mL trypsin (0.25% Trypsin+0.02% EDTA); ② Under the microscope, observe the digestion process. When the cell edge shrinks and the adhesion becomes loose (you can use a straw to suck up some trypsin and gently blow it onto a certain part of the cell layer. The cell layer can be seen to have fallen off with the naked eye, indicating that digestion is complete. Otherwise, continue digestion). Directly suck out the trypsin, add 5-6mL of complete culture medium, gently blow the cell layer, blow it off, and disperse. ③ Divide the cell suspension into new T25 bottles at a ratio of 1:2, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator.; ④ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80% -90%.
morphology	Epithelial cell like, polygonal, irregular edges, single-layer adhesion growth
Sharing mode	Public welfare sharing