Mouse mast cell tumor cells|100552 |BNCC

Essential Information Related Products

P815

Culture medium	BNCC338068
Description	DMEM-H Complete Medium (with 10% FBS)
Composition	90%DMEM-H+10%FBS
Growth conditions	37°C; 5% CO₂ + 95% air
Attention	If any abnormality is found on the day of receipt, please contact customer service within 24 hours. Failure to do so will be considered as confirmation of good condition upon receipt. For cryovial format: Upon receipt, immediately store at -80°C. For long-term storage, transfer to liquid nitrogen overnight. During resuscitation, use the entire contents of one vial at a time; do not reserve any portion. For T25 flask format: Upon receipt, let the flask stand in the incubator for 2–3 hours before proceeding with routine cell handling. Under sterile conditions, transfer the original culture medium from the flask to a centrifuge tube. Centrifuge at 1000–1200 rpm for 5–10 minutes, then resuspend the cells. Transfer the suspended cells back to the original T25 flask, add complete medium to a total volume of 10 mL, and place the flask in the incubator for cultivation. Please strictly follow the instructions above. Failure to do so may result in cell inactivation, and no replacement will be provided.
Subculture procedure	Recovery steps: ① Remove the cryovial from liquid nitrogen or -80 ℃ freezer and place it in PE gloves. Quickly immerse it in a 37 ℃ water bath and shake the cryovial to accelerate dissolution. It is recommended to dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete culture medium on a super clean bench, centrifuge at 1000-1200rpm for 5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete culture medium. ③ Then add the cell suspension to a T25 bottle containing 6-8mL of complete culture medium and culture it in an incubator.Cell passage: ① Gently scrape off the adherent cells with a cell scraper, transfer them along with the culture medium to a centrifuge tube, centrifuge at 1000 RPM for 5-8 minutes, and discard the supernatant; ② Add 1-2 mL of complete culture medium to the cell pellet collected from suspended cells and adherent cells, and mix well; ③ Add culture medium at a rate of 5-6mL/bottle, and divide the cell suspension into culture bottles containing 5-6mL of culture medium at a ratio of 1:2; ④ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80% -90%.
morphology	Lymphoblast-like，Spindle-shaped (short)，Round，irregular edge
Sharing mode	Public welfare sharing