Human non-small cell lung cancer cells|359250 |BNCC

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NCI-H1944

Culture medium	BNCC338360
Description	RPMI-1640 Complete Medium (with 10% FBS)
Composition	90%RPMI-1640+10%FBS
Growth conditions	37°C; 5% CO₂ + 95% air
Subculture procedure	Resuscitation steps: ① Remove the Frozen vial from liquid nitrogen or -80 ℃ and place it in a PE glove. Quickly immerse it in a 37 ℃ water bath, shake the Frozen vial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9ml of complete culture medium on a super clean bench, centrifuge at 1000-1200rpm/min for 5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete culture medium. ③ Then add the cell suspension to a T25 bottle containing 5-6mL of complete culture medium and culture it in an incubator. Cell passage: ① Remove the old culture medium, wash twice with PBS, and add 1-2mL Trypsin (0.25% Trypsin + 0.02% EDTA); ② Observe the digestion process under the microscope. When the cell edge shrinks and the adhesion becomes loose (you can use a straw to suck up some trypsin and gently blow it to a certain part of the cell layer. The cell layer can be seen to have fallen off with the naked eye, indicating that digestion is complete. Otherwise, continue digestion). Directly suck out the trypsin, add 5-6 milliliters of complete culture medium, gently blow the cell layer, blow it off, and disperse. ③ Divide the cell suspension into new T25 bottles at a ratio of 1:2, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator. ④ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80% -90%.
morphology	Epithelial-like，irregular edge，Monolayer adherent growth
Sharing mode	Public welfare sharing