Mouse monocyte macrophage leukemia cells (SQSTM1 gene marker)|393482 |BNCC

Essential Information Related Products

RAW264.7-SQSTM1

Growth conditions	37°C; 5% CO₂ + 95% air
Attention	If any abnormality is found on the day of receipt, please contact customer service within 24 hours. Failure to do so will be considered as confirmation of good condition upon receipt.For cryovial format: Upon receipt, immediately store at -80°C. For long-term storage, transfer to liquid nitrogen overnight. During resuscitation, use the entire contents of one vial at a time; do not reserve any portion.For T25 flask format: Upon receipt, let the flask stand in the incubator for 2–3 hours before proceeding with routine cell handling.Please strictly follow the instructions above. Failure to do so may result in cell inactivation, and no replacement will be provided.
Subculture procedure	Resuscitation steps: ① Remove the frozen vial from liquid nitrogen or -80 ℃ refrigerator and place it in PE gloves. Quickly immerse it in a 37 ℃ water bath, shake the vial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete medium on a ultra-clean bench, centrifuge at 1000-1200rpm for 5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete medium. ③ Add the cell suspension into a T25 flask containing 5-6mL of complete medium and place the flask in an incubator. Cell passage: ① Use a 5ml pipette to directly aspirate the culture medium and blow the cells; ② Observe under the microscope whether the cells are evenly dispersed. ③ Divide the cell suspension into fresh T25 flasks at a ratio of 1:2, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator.; ④ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80% -90%.
morphology	Macrophage-like，Round，Spindle-shaped (short)，Monolayer adherent growth
Sharing mode	Public welfare sharing