Escherichia coli phage MS2 nucleic acid reference product is available on the market!

At present, the PCR method based on molecular biology technology has become the main method for the detection of foodborne viruses, and its sensitivity and accuracy have been continuously improved. However, there are still factors such as operational errors and unknown nucleic acid extraction efficiency, which affect the reliability of the detection results. and comparability, which is not conducive to the standardization of detection methods. Therefore, in actual detection, each detection step needs to be strictly controlled. Escherichia coliphage MS2 can be used as a quality control substance in the molecular biology detection process of RNA viruses, and it has the following two advantages: (1) Phage is an RNA virus that invades bacteria. It has strict host specificity and only infects specific host bacteria. This is determined by the molecular structure complementarity between the phage adsorption organ and the receptor on the surface of the recipient bacteria. No harm to humans and animals, biosafety can meet the requirements of microbial reference materials; (2) E. coli phage MS2 (coliphage MS2) can be prepared at high concentrations with Escherichia coli as the host, and the concentration can reach 10^10~10^12 PFU/mL, It is more convenient to prepare.

The coliphage MS2 nucleic acid reference product developed by BNCC belongs to the heat-inactivated coliphage MS2, which is a virus particle that loses the growth and infection and pathogenicity of the phage through high temperature, but retains the complete genetic material. Mainly used for quality control substances in the molecular biology detection process of RNA viruses, positive controls for nucleic acid detection, etc.

Instruction:

This reference product is not recommended to be repeatedly frozen and thawed. Before use, it should be taken out of the refrigerator, equilibrated to room temperature, mixed well, and ready to use.

Notice:

1  After receiving the goods, please keep them properly. In case of damage, please contact us within 24 hours.

2 This product uses heat inactivation method, which is likely to cause protein damage, and is only suitable for nucleic acid and other tests.