The number of confirmed cases of monkeypox worldwide has exceeded 50,000! BNCC Monkeypox Virus Nucleic Acid Detection Kit Faces Challenges.

Monkeypox is currently breaking out all over the world, and mankind is facing the challenge of a global epidemic. Since May of this year, outbreaks of monkeypox have begun in many places around the world, and have spread from Europe and the United States to many places in Asia. Among them, Hong Kong confirmed the first importation of monkeypox on September 6. Under the new crown epidemic, monkeypox has been killed again, and the world is bound to face the challenge of double epidemic.

Monkeypox is a zoonotic viral disease caused by monkeypox virus infection. The main clinical manifestations are fever, rash, and swollen lymph nodes. At present, there is no universal, safe and rapid serological method for the diagnosis of monkeypox virus infection in the world. The monkeypox virus nucleic acid detection kit independently developed by BNCC designs specific detection primers and probes according to the specific gene sequence of monkeypox virus, and uses qPCR technology for detection. It has high sensitivity, strong specificity, easy operation, and accurate results. 

The "Guidelines for the Diagnosis and Treatment of Monkeypox (2022 Edition)" issued by the National Health and Medical Commission clearly stated that monkeypox virus can be detected in specimens such as rashes, blister fluid, scabs, oropharyngeal or nasopharyngeal secretions using nucleic acid amplification detection methods. nucleic acid. The monkeypox virus pseudovirus standard quality control sample can be used as a measurement standard for the development and performance evaluation of monkeypox virus detection kits.

Instruction:

Monkeypox virus nucleic acid detection kit

preparation:

dissolve the various components in the kit on ice before use, mix slightly and centrifuge briefly, and place on ice for later use.

reaction system (25 μL):18 μL of qPCR mixed reaction solution, 2 μL of template, 5μL of sterile water.

Reaction conditions: the first step is 95°C for 1min, the second step is 95°C for 10s, the third step is 55°C for 30s, and the second to third steps are cycled 40 times;

Fluorescence channel: FAM.

Monkeypox virus pseudovirus standard quality control product: Quality control products should be taken out of the refrigerator and equilibrated to room temperature before use. After it is completely melted, relevant experimental operations can be carried out. Nucleic acid extraction and purification reagents need to be prepared by themselves. The minimum sampling volume is 200μL. After the product is thawed, it should be used up once. Repeated freezing and thawing is not recommended.

Notice:

1  After receiving the goods, please keep them properly. In case of damage, please contact us within 24 hours.

2  Please operate in a biological safety cabinet in a sterile environment to avoid repeated freezing and thawing.

3   When using the standard, attention should be paid to biosafety protection, and it should be treated as potentially bioinfectious samples. The operation and treatment should comply with relevant regulations.